Enzyme immunoassay for diagnosis of gonorrhea

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Enzyme immunoassay for diagnosis of gonorrhea.

An enzyme immunoassay (EIA; Gonozyme [Abbott Laboratories]) for gonococcal antigen was assessed for the rapid diagnosis of gonorrhea. Patients attending two sexually transmitted disease clinics were tested by EIA and culture on Thayer-Martin medium. EIA was highly effective in detecting gonococcal infection among symptomatic men, with 70 of 75 (93.3%) culture-positive men having positive tests ...

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Evaluation of Gonozyme, an enzyme immunoassay for the rapid diagnosis of gonorrhea.

A new indirect enzyme immunoassay (EIA), Gonozyme (Abbott Laboratories), was assessed for rapid detection of gonococcal antigens. A correlation of optic density (OD) readings by EIA with colony counts of serial dilutions of Neisseria gonorrhoeae ATCC 19424 disclosed that EIA detected 10(3) CFU/ml at OD readings of 0.1 to 0.3, that EIA consistently detected greater than or equal to 10(4) CFU/ml ...

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Methodofluorescence for the Diagnosis of Gonorrhea

1. A direct fluorescent antibody (F.A.) tech­nique for diagnosis of Gonococci is de­scribed. 2. The results of applying F.A. method in the 270 female cases are presented and compared with the conventional method find­ings. 3. 220 cases were diagnosed by one or more methods at the first examinations. F .A. tests alone were positive in 44 ,eases and .F .A. direct diagnosis was made in a tot...

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Comparison of Enzyme Immunoassay, Immunochromatography, and RNA-Polyacrylamide-Gel Electrophoresis for Diagnosis of Rotavirus Infection in Children with Acute Gastroenteritis

Human rotavirus is a major etiologic agent for infantile diarrhea worldwide. It is responsible for up to 3.3 million deaths per year in children in developing countries. Various rapid and sensitive techniques have been developed to readily diagnose rotavirus gastroenteritis. In the present study, we compared the sensitivity and specificity of immunochromatography and RNA-polyacrylamide-gel elec...

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Efficacy of an enzyme immunoassay with uncentrifuged first-voided urine for detection of gonorrhea in males.

An enzyme immunoassay (Gonozyme; Abbott Laboratories, North Chicago, Ill.) for detection of Neisseria gonorrhoeae antigens was used to screen 184 urethral or uncentrifuged first-voided urine or both specimens from males and 78 cervical specimens. When compared with culture, the sensitivity and specificity of Gonozyme for cervical and urethral specimens were comparable to those in published repo...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1984

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.19.1.57-59.1984